Plant Embryo Culture: Methods and Protocols (Methods in Molecular Biology)

Description: A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book's main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology(TM) series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes. Part I: Culture of Zygotic Embryos: Fundamental Studies 1. Zygotic Embryo Culture: An Overview Tegan M. Haslam and Edward C. Yeung 2. In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture Takashi Okamoto 3. Canola Zygotic Embryo Culture Nicole S. Ramesar-Fortner and Edward C. Yeung 4. Immature Seeds and Embryos of Medicago truncatula Cultured In vitro Sergio J. Ochatt 5. In vitro Culture and Germination of Terrestrial Asian Orchid Seeds Yung-I Lee 6. In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos Florent Engelmann, Bernard Malaurie, and Oulo N'Nan Part II: Application of Embryo Culture T echniques: Embryo R escue 7. Immature Embryo Rescue and Culture Xiuli Shen, Fred G. Gmitter, Jr., and Jude W. Grosser 8. Chickpea Hybridization Using In vitro Techniques Nalini Mallikarjuna and Fred J. Muehlbauer 9. Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, and Neftalí Ochoa-Alejo 10. Phaseolus Immature Embryo Rescue Technology Pascal Geerts, André Toussaint, Guy Mergeai, and Jean-Pierre Baudoin 11. Wide Crossing in Lentil through Embryo Rescue Richard Fratini and Maria L. Ruiz 12. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques Ajoy Kumar Roy, Devendra Ram Malaviya, and Pankaj Kaushal Part III: Cryopreservation of Embryos 13. Cryopreservation of Embryos: An Overview Florent Engelmann 14. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm Anna De Carlo, Maurizio Lambardi, and Elif Aylin Ozudogru 15. Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut Ana M. Vieitez, M. Carmen San-José, and Elena Corredoira 16. Cryopreservation of Ilex Immature Zygotic Embryos Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, and Hebe Rey Part IV: The Use of Zygotic Embryos as Explants for Somatic Embryogenesis and/or Organogenesis 17. The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview Mohamed Elhiti and Claudio Stasolla 18. Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos Malgorzata D. Gaj 19. Pine Somatic Embryogenesis Using Zygotic Embryos as Explants Gerald S. Pullman and Kylie Bucalo 20. Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies Kee Yoeup Paek, Eun Joo Hahn, and So Young Park Part V: Transformation U sing Zygotic Embryos as Explants 21. Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview Muhammad Tahir, Ejaz A. Waraich, and Claudio Stasolla 22. Genetic Transformation Using Maize Immature Zygotic Embryos Bronwyn Frame, Marcy Main, Rosemarie Schick, and Kan Wang 23. Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue Martha M. O'Kennedy, Hester C. Stark, and Nosisa Dube 24. Agrobacterium tumefaciens -Mediated Genetic Transformation of Cereals Using Immature Embryos Ashok K. Shrawat and Allen G. Good

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Location: Hillsdale, NSW

End Time: 2024-12-04T17:22:00.000Z

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